Serves as a senior researcher with expertise in field. Plans, designs and conducts highly technical and complex research projects independently and in consultation with supervisor, which require the regular use of ingenuity and innovation. May contribute to the development of new concepts, techniques and standards. Exercises considerable latitude in determining research objectives and approaches.
Identifies, researches, compiles and evaluates data sources, background information and/or technology related to area of specialization.
Analyzes and evaluates research data utilizing computers and provides interpretations requiring significant knowledge of a specialized area of research. Searches literature, utilizing all available resources including electronic, regarding new methodology and designs experiments accordingly.
Develops solutions to complex research problems, which require the regular use of ingenuity and innovation. Ensures solutions are consistent with research objectives.
Contributes to the development of research documentation for publication and/or prepares technical reports, papers and/or records.
Operates and maintains sophisticated laboratory/scientific equipment.
Quantitative Real-time RT-PCR reaction to analyze the change of expression level of mRNA in kidney tissue.
Laser capturing micro-dissection to isolate RNA and protein from individual glomeruli in kidney.
Flow cytometry staining and analysis on digested kidney tissue for isolated leukocytes, splenocytes, blood leukocytes, and peritoneal macrophages.
Magnetic bead isolation and enrichment of macrophages from digested and filtered kidney tissue.
Double staining for immunohistochemistry and immunofluorescence;
Cell culture of glomerular mesangial cells and peritoneal macrophages.
Western blotting and enzyme-linked immunosorbent assays.
Morphometric analysis to quantify pathological changes in kidney tissue.
Mouse breeding, genotyping, and I.V. tail vein injection.
Performs other related duties as assigned or requested. The University reserves the right to add or change duties at any time.
The University of Southern California values diversity and is committed to equal opportunity in employment.
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